Indirect Determination of Ascorbic Acid by Atomic Absorption Spectroscopy

Document Type : Research Paper

Authors

• Masoomeh Emadi ¹
• Mohammad AliZare Zare ²
• Omran Moradlou ³
• Maryam Iranpour ⁴

¹ Young Researchers and Elite Club, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

² 1Young Researchers and Elite Club, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran

³ 2Department of Chemistry, College of Sciences, Alzahra University, Tehran, Iran

⁴ Department of Science and Research Branch, Islamic Azad University, Fars, Iran

Abstract

A new and simple method followed by atomic absorption spectrometry has been developed
for indirect determination of ascorbic acid (AA). The proposed method was based on the oxidation
of AA to dehydroascorbic acid with Cu2+cation(200mgL-1)in an ammonium thiocyanatesolution at
pH2.4. AA reduces Cu2+to Cu+followed by the precipitation of Cu+ with SCN-. Then, the excess of
Cu2+in the solution was measured by the atomic absorption spectrometry.The results showed that
AA can be sensitively measured in the linear range of 2.0 to 40.0 mgL-1 with the detection limit of
1.5mgL-1.The relative standard deviation (%RSD) was 0.319 in 7 determination of 2.0 mgL-1 AA.
Finally, the method was successfully used for the determination of AA in tablets containing various
amounts of AA.

Keywords

• Ascorbic acid
• indirect determination
• atomic absorption spectroscopy

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